L-929小鼠成纤维细胞L92

  特别提醒：该产品仅限于实验室科学研究使用，不得用于其他用途 细胞名称：NCTC clone 929 [L cell, L-929, derivative of Strain L]     小鼠成纤维细胞组织来源：皮下结缔组织；疏松结缔组织及脂肪培养条件：MEM +10% FBS形      态：贴壁；成纤维细胞样背      景：1948年3月建立了NCTC clone 929(小鼠结缔组织)，细胞系L的克隆。细胞系L是最早建立的连续培养细胞系之一，而clone 929是最早的克隆株。从一只100日龄的雄性C3H/An小鼠的皮下网眼状空隙和脂肪组织中建立了亲本细胞系L。第95代的细胞系L使用毛细管法分离单细胞建立了clone929。检测发现鼠痘病毒阴性。     Group: Space-flown cell line (cellonaut).Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).Virology: Not susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19) (PubMed=33389257).Doubling time: 28 hours (Note=From cell counting), 32 hours (Note=From absorbance) (DOI=10.5897/IJBMBR2013.0154); ~25 hours (CLS=400260); ~21-24 hours (DSMZ=ACC-2).Omics: Deep quantitative proteome analysis.Anecdotal: First established continuous cell line.Miscellaneous: PubMed=23430347 has a different value for STR 6-4 (16) than that of NIST (17,18) due to a change in the marker motif (personal communication of Almeida J.L.).Derived from site: In situ; Connective tissue; UBERON=UBERON_0002384.Cell type: Fibroblast; CL=CL_0000057.